Selected Reaction Monitoring
Figure 1. Examples of the analysis of two proteins, catalase and acyl CoA oxidase, in liver. Each protein is measured via two peptides detected in the SRM experiment.
Kinter, C.S., Lundie, J.M., Patel, H., Rindler, P.M., Szweda, L.I., and Kinter, M. (2102) A quantitative proteomic profile of the Nrf2-mediated antioxidant response of macrophages to oxidized LDL determined by multiplexed selected reaction monitoring. PLoS One. 7, e50016.
Kinter, M. and Sherman, N.E. (2000) Protein Sequencing and Identification Using Tandem Mass Spectrometry. Wiley, New York.
Kinter, M. and Kinter, C.S. (2013) Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics: A Replacement for Western Blot. SpringerBriefs, New York.
Ludwig, C., Claassen, M., Schmidt, A., and Aebersold, R. (2012) Estimation of absolute protein quantities of unlabeled samples by selected reaction monitoring mass spectrometry. Mol. Cell. Proteomics. 11, M111.013987.
Contact Mike Kinter (firstname.lastname@example.org) prior to submitting samples. The goal of the contact will be to develop a detailed plan for the analyses, including:
Our procedures require at least 150µg of protein per sample that is submitted. Samples are submitted as homogenates that are ready for our protein assay. Our protein assay will use approximately 1/3rd of the sample, which is required for an accurate assay of the proteins in the sample. Samples should be clearly labeled with an informative sample name. We will carry these names through our notes, logs, and reports. Below are examples of methods we have the most experience using.